Hotspot

psbC

LSC · 33909-35330 · 1422 bp

Back to Acrocomia

Pi 0

MarkerSeek score 31.10

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTTCCCAAGAAATCCGTGCA	CTAGCCGAGCCAAAACAGAA	45.0	50.0	58.0	58.2	1586 bp	1.0	78.4
psbC_p2	TTTCCCAAGAAATCCGTGCA	GCCGAGCCAAAACAGAAAAA	45.0	45.0	58.0	57.8	1583 bp	1.0	78.2
psbC_p3	TTTCCCAAGAAATCCGTGCA	TAGCCGAGCCAAAACAGAAA	45.0	45.0	58.0	57.7	1585 bp	1.0	77.8
psbC_p4	GCAGCGGAAGATCCTGAATT	CTAGCCGAGCCAAAACAGAA	50.0	50.0	58.3	58.2	1569 bp	1.0	75.9
psbC_p5	AGGTATTCGTGCTTGGATGG	CTAGCCGAGCCAAAACAGAA	50.0	50.0	57.7	58.2	1513 bp	1.0	75.8