Hotspot

matK

LSC · 1723-3270 · 1548 bp

Back to Acrocomia

Pi 0.000646

MarkerSeek score 59.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1548
variable_sites: 1
pi: 0.000646
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCTCATCACGTCACCAAACC	TCCTGCCTCTGGTTCAAGTA	50.0	50.0	57.8	58.0	1599 bp	1.0	78.5
matK_p2	TCTCATCACGTCACCAAACC	AATGAAATGGGTCCTGCCTC	50.0	50.0	57.8	57.9	1610 bp	1.0	77.5
matK_p3	TCTCATCACGTCACCAAACC	CCTGCCTCTGGTTCAAGTAG	50.0	55.0	57.8	57.6	1598 bp	1.0	75.6
matK_p4	TCTCATCACGTCACCAAACC	AAATGAAATGGGTCCTGCCT	50.0	45.0	57.8	57.4	1611 bp	1.0	73.7
matK_p5	TCTCATCACGTCACCAAACC	ATGAAATGGGTCCTGCCTCT	50.0	50.0	57.8	58.7	1609 bp	1.0	73.2