Hotspot

accD

LSC · 58610-60085 · 1476 bp

Back to Acrocomia

Pi 0.000678

MarkerSeek score 59.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1476
variable_sites: 1
pi: 0.000678
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATAGGGGGCAAGAAGACTCT	ACGATGAACTAAATACTTGTTCGC	50.0	37.5	57.4	57.9	1551 bp	1.0	42.7
accD_p2	ATAGGGGGCAAGAAGACTCT	CGATGAACTAAATACTTGTTCGCT	50.0	37.5	57.4	57.7	1550 bp	1.0	40.8
accD_p3	ATAGGGGGCAAGAAGACTCT	ACTTGTTCGCTACAAATAACTGA	50.0	34.8	57.4	56.5	1537 bp	1.0	40.1
accD_p4	ATAGGGGGCAAGAAGACTCT	CGATGAACTAAATACTTGTTCGC	50.0	39.1	57.4	56.4	1550 bp	1.0	40.1
accD_p5	AATAGGGGGCAAGAAGACTCT	ACGATGAACTAAATACTTGTTCGC	47.6	37.5	58.1	57.9	1552 bp	1.0	40.1