Hotspot

psaJ-rpl33

LSC · 67879-68430 · 552 bp

Back to Acrocomia

Pi 0
MarkerSeek score 57.90
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
552
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TGGTTTGGGTCTTTAGCAGG TGACACCATTTCGGACACAA 50.0 45.0 57.7 57.7 705-706 bp 1.0 75.2
psaJ-rpl33_p2 TGGTTTGGGTCTTTAGCAGG TTGACACCATTTCGGACACA 50.0 45.0 57.7 57.7 706-707 bp 1.0 75.2
psaJ-rpl33_p3 GGTTTGGGTCTTTAGCAGGT TGACACCATTTCGGACACAA 50.0 45.0 57.7 57.7 704-705 bp 1.0 75.1
psaJ-rpl33_p4 GGTTTGGGTCTTTAGCAGGT TTGACACCATTTCGGACACA 50.0 45.0 57.7 57.7 705-706 bp 1.0 75.1
psaJ-rpl33_p5 TGGTTTGGGTCTTTAGCAGG ACACCATTTCGGACACAACT 50.0 45.0 57.7 57.7 703-704 bp 1.0 75.1