Hotspot

trnP-UGG-psaJ

LSC · 67337-67743 · 407 bp

Back to Acrocomia

Pi 0.00246

MarkerSeek score 56.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 407
variable_sites: 1
pi: 0.00246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ATCCCTCGTTACTGCCCATA	CCTGCTAAAGACCCAAACCA	50.0	50.0	57.9	57.7	586 bp	1.0	77.1
trnP-UGG-psaJ_p2	ATCCCTCGTTACTGCCCATA	ACCTGCTAAAGACCCAAACC	50.0	50.0	57.9	57.7	587 bp	1.0	77.1
trnP-UGG-psaJ_p3	ACCCGTGACATTTTGTACCC	CCTGCTAAAGACCCAAACCA	50.0	50.0	58.1	57.7	533 bp	1.0	77.1
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	CCTGCTAAAGACCCAAACCA	50.0	50.0	58.1	57.7	532 bp	1.0	77.1
trnP-UGG-psaJ_p5	ACCCGTGACATTTTGTACCC	ACCTGCTAAAGACCCAAACC	50.0	50.0	58.1	57.7	534 bp	1.0	77.0