primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TGCCAATGTCAACCAATCTCA	GTCGTGCAGTGCAATTCAAT	21	20	42.9	45.0	58.1	57.7	0.0	21.7	1.462541	1416	4109	2870-2870	1.000000	68.3
trnK-UUU_p2	trnK-UUU	2	TGCCAATGTCAACCAATCTCA	TCAATGTTTTGACGGGGTCG	21	20	42.9	50.0	58.1	58.8	0.0	0.0	1.894766	1416	4109	2886-2886	1.000000	64.9
trnK-UUU_p3	trnK-UUU	3	GCCAATGTCAACCAATCTCA	GTCGTGCAGTGCAATTCAAT	20	20	45.0	45.0	56.3	57.7	0.0	21.7	2.028443	1416	4109	2869-2869	1.000000	63.8
trnK-UUU_p4	trnK-UUU	4	TGCCAATGTCAACCAATCTC	GTCGTGCAGTGCAATTCAAT	20	20	45.0	45.0	56.3	57.7	0.0	21.7	2.028443	1416	4109	2870-2870	1.000000	63.8
trnK-UUU_p5	trnK-UUU	5	TGCCAATGTCAACCAATCTCA	GTTCAATGTTTTGACGGGGTC	21	21	42.9	47.6	58.1	58.0	0.0	0.0	2.133966	1416	4109	2888-2888	1.000000	63.0
matK_p1	matK	1	TCTCATCACGTCACCAAACC	TCCTGCCTCTGGTTCAAGTA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.187994	1723	3270	1599-1599	1.000000	78.5
matK_p2	matK	2	TCTCATCACGTCACCAAACC	AATGAAATGGGTCCTGCCTC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.313791	1723	3270	1610-1610	1.000000	77.5
matK_p3	matK	3	TCTCATCACGTCACCAAACC	CCTGCCTCTGGTTCAAGTAG	20	20	50.0	55.0	57.8	57.6	0.0	0.0	0.557089	1723	3270	1598-1598	1.000000	75.6
matK_p4	matK	4	TCTCATCACGTCACCAAACC	AAATGAAATGGGTCCTGCCT	20	20	50.0	45.0	57.8	57.4	0.0	0.0	0.792835	1723	3270	1611-1611	1.000000	73.7
matK_p5	matK	5	TCTCATCACGTCACCAAACC	ATGAAATGGGTCCTGCCTCT	20	20	50.0	50.0	57.8	58.7	0.0	0.0	0.860911	1723	3270	1609-1609	1.000000	73.2
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	GGATCCTAGTTCGGGACTGA	AATTCTTCCTGGGTCGATGC	20	20	55.0	50.0	57.9	58.0	3.8	0.0	0.112957	30904	31334	610-610	1.000000	79.2
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	GTGCTCTGACCGATTGAACT	AATTCTTCCTGGGTCGATGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.130177	30904	31334	553-553	1.000000	79.1
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	GGATCCTAGTTCGGGACTGA	GGGGACGGACTGTAAATTCG	20	20	55.0	55.0	57.9	58.4	3.8	0.0	0.420330	30904	31334	577-577	1.000000	76.8
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	ATCGGATCCTAGTTCGGGAC	AATTCTTCCTGGGTCGATGC	20	20	55.0	50.0	58.4	58.0	10.8	0.0	0.425635	30904	31334	613-613	1.000000	76.7
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CATCGGATCCTAGTTCGGGA	AATTCTTCCTGGGTCGATGC	20	20	55.0	50.0	58.4	58.0	10.8	0.0	0.426800	30904	31334	614-614	1.000000	76.7
psbC_p1	psbC	1	TTTCCCAAGAAATCCGTGCA	CTAGCCGAGCCAAAACAGAA	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.203296	33909	35330	1586-1586	1.000000	78.4
psbC_p2	psbC	2	TTTCCCAAGAAATCCGTGCA	GCCGAGCCAAAACAGAAAAA	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.229136	33909	35330	1583-1583	1.000000	78.2
psbC_p3	psbC	3	TTTCCCAAGAAATCCGTGCA	TAGCCGAGCCAAAACAGAAA	20	20	45.0	45.0	58.0	57.7	0.0	0.0	0.275712	33909	35330	1585-1585	1.000000	77.8
psbC_p4	psbC	4	GCAGCGGAAGATCCTGAATT	CTAGCCGAGCCAAAACAGAA	20	20	50.0	50.0	58.3	58.2	0.6	0.0	0.518421	33909	35330	1569-1569	1.000000	75.9
psbC_p5	psbC	5	AGGTATTCGTGCTTGGATGG	CTAGCCGAGCCAAAACAGAA	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.523652	33909	35330	1513-1513	1.000000	75.8
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.188901	35331	35467	283-283	1.000000	79.3
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	35331	35467	244-244	1.000000	79.2
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	CTGTTCTTTCCATGACCCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	35331	35467	243-243	1.000000	79.2
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	GCAGCAGGGTTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.259146	35331	35467	294-294	1.000000	78.7
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	CTGTTCTTTCCATGACCCCC	GATGGCCGAGTGGTTGATAG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.289496	35331	35467	254-254	1.000000	78.6
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	48395	49111	827-831	1.000000	78.7
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	48395	49111	817-821	1.000000	78.1
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGAAATGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.300111	48395	49111	873-877	1.000000	77.7
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	48395	49111	870-874	1.000000	77.4
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	48395	49111	869-873	1.000000	77.4
accD_p1	accD	1	ATAGGGGGCAAGAAGACTCT	ACGATGAACTAAATACTTGTTCGC	20	24	50.0	37.5	57.4	57.9	0.0	0.0	4.668895	58610	60085	1551-1551	1.000000	42.7
accD_p2	accD	2	ATAGGGGGCAAGAAGACTCT	CGATGAACTAAATACTTGTTCGCT	20	24	50.0	37.5	57.4	57.7	0.0	0.0	4.910276	58610	60085	1550-1550	1.000000	40.8
accD_p3	accD	3	ATAGGGGGCAAGAAGACTCT	ACTTGTTCGCTACAAATAACTGA	20	23	50.0	34.8	57.4	56.5	0.0	4.8	5.084010	58610	60085	1537-1537	1.000000	40.1
accD_p4	accD	4	ATAGGGGGCAAGAAGACTCT	CGATGAACTAAATACTTGTTCGC	20	23	50.0	39.1	57.4	56.4	0.0	0.0	5.208856	58610	60085	1550-1550	1.000000	40.1
accD_p5	accD	5	AATAGGGGGCAAGAAGACTCT	ACGATGAACTAAATACTTGTTCGC	21	24	47.6	37.5	58.1	57.9	0.0	0.0	5.238993	58610	60085	1552-1552	1.000000	40.1
accD-psaI_p1	accD-psaI	1	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	60086	60716	741-741	1.000000	80.0
accD-psaI_p2	accD-psaI	2	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	60086	60716	742-742	1.000000	80.0
accD-psaI_p3	accD-psaI	3	CCACGGTTTCTTTCCCTTGA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.127877	60086	60716	747-747	1.000000	79.4
accD-psaI_p4	accD-psaI	4	TCCACGGTTTCTTTCCCTTG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.127877	60086	60716	748-748	1.000000	79.4
accD-psaI_p5	accD-psaI	5	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	0.0	26.9	0.148050	60086	60716	747-747	1.000000	79.2
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.350824	63840	64853	1165-1166	1.000000	78.4
petA-psbJ_p2	petA-psbJ	2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.466931	63840	64853	1161-1162	1.000000	77.4
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.467291	63840	64853	1148-1149	1.000000	77.4
petA-psbJ_p4	petA-psbJ	4	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.468635	63840	64853	1164-1165	1.000000	77.4
petA-psbJ_p5	petA-psbJ	5	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.2	58.3	13.8	0.0	0.491221	63840	64853	1162-1163	1.000000	77.2
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ATCCCTCGTTACTGCCCATA	CCTGCTAAAGACCCAAACCA	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.383475	67337	67743	586-586	1.000000	77.1
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	ATCCCTCGTTACTGCCCATA	ACCTGCTAAAGACCCAAACC	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.385087	67337	67743	587-587	1.000000	77.1
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACCCGTGACATTTTGTACCC	CCTGCTAAAGACCCAAACCA	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.386119	67337	67743	533-533	1.000000	77.1
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CCCGTGACATTTTGTACCCA	CCTGCTAAAGACCCAAACCA	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.387463	67337	67743	532-532	1.000000	77.1
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	ACCCGTGACATTTTGTACCC	ACCTGCTAAAGACCCAAACC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.387732	67337	67743	534-534	1.000000	77.0
psaJ-rpl33_p1	psaJ-rpl33	1	TGGTTTGGGTCTTTAGCAGG	TGACACCATTTCGGACACAA	20	20	50.0	45.0	57.7	57.7	0.0	0.0	0.620053	67879	68430	705-706	1.000000	75.2
psaJ-rpl33_p2	psaJ-rpl33	2	TGGTTTGGGTCTTTAGCAGG	TTGACACCATTTCGGACACA	20	20	50.0	45.0	57.7	57.7	0.0	0.0	0.620053	67879	68430	706-707	1.000000	75.2
psaJ-rpl33_p3	psaJ-rpl33	3	GGTTTGGGTCTTTAGCAGGT	TGACACCATTTCGGACACAA	20	20	50.0	45.0	57.7	57.7	0.0	0.0	0.621666	67879	68430	704-705	1.000000	75.1
psaJ-rpl33_p4	psaJ-rpl33	4	GGTTTGGGTCTTTAGCAGGT	TTGACACCATTTCGGACACA	20	20	50.0	45.0	57.7	57.7	0.0	0.0	0.621666	67879	68430	705-706	1.000000	75.1
psaJ-rpl33_p5	psaJ-rpl33	5	TGGTTTGGGTCTTTAGCAGG	ACACCATTTCGGACACAACT	20	20	50.0	45.0	57.7	57.7	0.0	0.0	0.628211	67879	68430	703-704	1.000000	75.1
rpl14_p1	rpl14	1	TGCGTGAGACACAATCTATT	TCCTTCATCCTTTGCATTGA	20	20	40.0	40.0	54.8	54.9	0.0	0.0	6.277922	81705	82073	486-486	1.000000	40.0
rpl14_p2	rpl14	2	TGCGTGAGACACAATCTATT	TCCTTCATCCTTTGCATTGAA	20	21	40.0	38.1	54.8	55.7	0.0	0.0	6.445635	81705	82073	486-486	1.000000	40.0
rpl14_p3	rpl14	3	TGCGTGAGACACAATCTATT	TTCCTTCATCCTTTGCATTGA	20	21	40.0	38.1	54.8	55.7	0.0	0.0	6.445635	81705	82073	487-487	1.000000	40.0
rpl14_p4	rpl14	4	ATATGCGTGAGACACAATCT	TCCTTCATCCTTTGCATTGA	20	20	40.0	40.0	54.6	54.9	0.0	0.0	6.514550	81705	82073	489-489	1.000000	40.0
rpl14_p5	rpl14	5	ATGCGTGAGACACAATCTAT	TCCTTCATCCTTTGCATTGA	20	20	40.0	40.0	54.6	54.9	0.0	0.0	6.514550	81705	82073	487-487	1.000000	40.0
rpl16_p1	rpl16	1	TCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	20	26	40.0	30.8	54.9	58.1	0.0	0.0	9.192583	82205	83734	1676-1677	1.000000	40.1
rpl16_p2	rpl16	2	TCAATGCAAAGGATGAAGGA	GGTTGAATAAAAATTCGATTGACCA	20	25	40.0	32.0	54.9	56.9	0.0	0.0	9.198462	82205	83734	1675-1676	1.000000	40.1
rpl16_p3	rpl16	3	TCAATGCAAAGGATGAAGGAA	AGGTTGAATAAAAATTCGATTGACCA	21	26	38.1	30.8	55.7	58.1	0.0	0.0	9.360297	82205	83734	1676-1677	1.000000	40.1
rpl16_p4	rpl16	4	TTCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	21	26	38.1	30.8	55.7	58.1	0.0	0.0	9.360297	82205	83734	1677-1678	1.000000	40.1
rpl16_p5	rpl16	5	TCAATGCAAAGGATGAAGGAA	GGTTGAATAAAAATTCGATTGACCA	21	25	38.1	32.0	55.7	56.9	0.0	0.0	9.366175	82205	83734	1675-1676	1.000000	40.1
ndhB_p1	ndhB	1	GTTCATAACCCCAGCCCATT	TTCGGAGATTGGATGCAGTT	20	20	50.0	45.0	57.8	57.2	0.0	0.0	0.946652	96179	98415	2371-2371	1.000000	72.5
ndhB_p2	ndhB	2	GTTCATAACCCCAGCCCATT	TTTCGGAGATTGGATGCAGT	20	20	50.0	45.0	57.8	57.2	0.0	0.0	0.946652	96179	98415	2372-2372	1.000000	72.5
ndhB_p3	ndhB	3	TTTGGGCGGAACAGATCTAC	TTCGGAGATTGGATGCAGTT	20	20	50.0	45.0	57.6	57.2	0.0	0.0	1.184063	96179	98415	2342-2342	1.000000	70.6
ndhB_p4	ndhB	4	TTTGGGCGGAACAGATCTAC	TTTCGGAGATTGGATGCAGT	20	20	50.0	45.0	57.6	57.2	0.0	0.0	1.184063	96179	98415	2343-2343	1.000000	70.6
ndhB_p5	ndhB	5	TTGGGCGGAACAGATCTACT	TTCGGAGATTGGATGCAGTT	20	20	50.0	45.0	58.4	57.2	0.0	0.0	1.218969	96179	98415	2341-2341	1.000000	70.3
ccsA_p1	ccsA	1	GGCGGCATCCTCTAAAAGAG	ACTCGAACAAGCAAACTTTCG	20	21	55.0	42.9	58.4	57.7	3.1	0.0	1.731691	115561	116529	1122-1122	1.000000	66.1
ccsA_p2	ccsA	2	GGCGGCATCCTCTAAAAGAG	CTCGAACAAGCAAACTTTCGT	20	21	55.0	42.9	58.4	57.7	3.1	0.0	1.731691	115561	116529	1121-1121	1.000000	66.1
ccsA_p3	ccsA	3	GGCGGCATCCTCTAAAAGAG	TCGAACAAGCAAACTTTCGT	20	20	55.0	40.0	58.4	56.5	3.1	0.0	1.891663	115561	116529	1120-1120	1.000000	64.9
ccsA_p4	ccsA	4	GCGGCATCCTCTAAAAGAGA	ACTCGAACAAGCAAACTTTCG	20	21	50.0	42.9	57.4	57.7	5.5	0.0	1.923284	115561	116529	1121-1121	1.000000	64.6
ccsA_p5	ccsA	5	GCGGCATCCTCTAAAAGAGA	CTCGAACAAGCAAACTTTCGT	20	21	50.0	42.9	57.4	57.7	5.5	0.0	1.923284	115561	116529	1120-1120	1.000000	64.6
ccsA-ndhD_p1	ccsA-ndhD	1	ACGAATTCGGCACTTATAGCT	CCCGATTTCGTTCTCTCGTT	21	20	42.9	50.0	57.5	58.0	29.8	0.0	1.462202	116530	116744	404-404	1.000000	68.9
ccsA-ndhD_p2	ccsA-ndhD	2	ACGAATTCGGCACTTATAGCT	ATCCCGATTTCGTTCTCTCG	21	20	42.9	50.0	57.5	57.5	29.8	0.0	1.948084	116530	116744	406-406	1.000000	65.0
ccsA-ndhD_p3	ccsA-ndhD	3	ACGAATTCGGCACTTATAGC	CCCGATTTCGTTCTCTCGTT	20	20	45.0	50.0	56.0	58.0	29.8	0.0	1.973377	116530	116744	404-404	1.000000	64.8
ccsA-ndhD_p4	ccsA-ndhD	4	ACGAATTCGGCACTTATAGCT	TCAGTTGACAGGGTACAAGC	21	20	42.9	50.0	57.5	57.5	29.8	0.0	1.998149	116530	116744	383-383	1.000000	64.6
ccsA-ndhD_p5	ccsA-ndhD	5	ACGAATTCGGCACTTATAGCT	CAGTTGACAGGGTACAAGCT	21	20	42.9	50.0	57.5	57.4	29.8	0.0	2.005202	116530	116744	382-382	1.000000	64.6
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	118615	119259	803-839	1.000000	77.6
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	118615	119259	802-838	1.000000	77.6
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	20	20	50.0	50.0	58.1	58.6	0.0	12.2	0.757229	118615	119259	823-859	1.000000	77.5
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	118615	119259	801-837	1.000000	76.5
psaC-ndhE_p5	psaC-ndhE	5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.943571	118615	119259	778-814	1.000000	76.2
ndhB_p1	ndhB	1	TTCGGAGATTGGATGCAGTT	GTTCATAACCCCAGCCCATT	20	20	45.0	50.0	57.2	57.8	0.0	0.0	0.946652	143234	145470	2371-2371	1.000000	72.5
ndhB_p2	ndhB	2	TTTCGGAGATTGGATGCAGT	GTTCATAACCCCAGCCCATT	20	20	45.0	50.0	57.2	57.8	0.0	0.0	0.946652	143234	145470	2372-2372	1.000000	72.5
ndhB_p3	ndhB	3	TTCGGAGATTGGATGCAGTT	TTTGGGCGGAACAGATCTAC	20	20	45.0	50.0	57.2	57.6	0.0	0.0	1.184063	143234	145470	2342-2342	1.000000	70.6
ndhB_p4	ndhB	4	TTTCGGAGATTGGATGCAGT	TTTGGGCGGAACAGATCTAC	20	20	45.0	50.0	57.2	57.6	0.0	0.0	1.184063	143234	145470	2343-2343	1.000000	70.6
ndhB_p5	ndhB	5	TTCGGAGATTGGATGCAGTT	TTGGGCGGAACAGATCTACT	20	20	45.0	50.0	57.2	58.4	0.0	0.0	1.218969	143234	145470	2341-2341	1.000000	70.3