Hotspot

rpl16

LSC · 80948-82294 · 1347 bp

Back to Acorus

Pi 0.00953
MarkerSeek score 54.90
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1347
variable_sites
21
pi
0.00953
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCTTTGTTTCCACACTCCTT ACCATTTCATTTGGTAGAAGTGC 40.0 39.1 54.7 57.6 1392-1528 bp 1.0 41.2
rpl16_p2 ATCTTTGTTTCCACACTCCT ACCATTTCATTTGGTAGAAGTGC 40.0 39.1 54.5 57.6 1393-1529 bp 1.0 41.2
rpl16_p3 TCTTTGTTTCCACACTCCTT CCATTTCATTTGGTAGAAGTGCT 40.0 39.1 54.7 57.4 1391-1527 bp 1.0 41.2
rpl16_p4 ATCTTTGTTTCCACACTCCTT ACCATTTCATTTGGTAGAAGTGC 38.1 39.1 55.3 57.6 1393-1529 bp 1.0 41.2
rpl16_p5 ACCCTTTATCTTTGTTTCCACAC ACCATTTCATTTGGTAGAAGTGC 39.1 39.1 57.2 57.6 1400-1536 bp 1.0 41.2