Hotspot

ndhF

SSC · 110030-112249 · 2220 bp

Pi 0.00893

MarkerSeek score 49.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 33
pi: 0.00893
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAAGACAACAGCTCAGGTCC	TATAGCCTCGTCGGAATCGA	55.0	50.0	58.2	57.8	2967-2989 bp	0.75	69.4
ndhF_p2	CGAGAAGACAACAGCTCAGG	TATAGCCTCGTCGGAATCGA	55.0	50.0	58.3	57.8	2970-2992 bp	0.75	68.7
ndhF_p3	TCGAGAAGACAACAGCTCAG	TATAGCCTCGTCGGAATCGA	50.0	50.0	57.3	57.8	2971-2993 bp	0.75	65.3
ndhF_p4	GCTCAGGTCCAGGACAGTTA	TATAGCCTCGTCGGAATCGA	55.0	50.0	58.7	57.8	2957-2979 bp	0.75	65.2
ndhF_p5	AGAAGACAACAGCTCAGGTC	TATAGCCTCGTCGGAATCGA	50.0	50.0	57.2	57.8	2968-2990 bp	0.75	64.4