Hotspot

atpE

LSC · 54000-54404 · 405 bp

Back to Acorus

Pi 0.014
MarkerSeek score 39.80
Primer available yes
Label atpE

Feature Metadata

label_name
atpE
feature_type
gene
region
LSC
length_bp
405
variable_sites
9
pi
0.014
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpE_p1 ACTCCCCTGAAAAGCATTGG ACCGCGAAGGCTATGAATTT 50.0 45.0 58.1 58.0 926-934 bp 1.0 81.6
atpE_p2 ACTCCCCTGAAAAGCATTGG ATCTTCCGGTTCGTACAAGC 50.0 50.0 58.1 58.0 1553-1561 bp 1.0 81.0
atpE_p3 ACTCCCCTGAAAAGCATTGG CCTGCGGATGATTTGACTGA 50.0 50.0 58.1 58.0 1388-1396 bp 1.0 81.0
atpE_p4 ACTCCCCTGAAAAGCATTGG TTGGTTTGACTGCCCTAACC 50.0 50.0 58.1 58.0 1630-1638 bp 1.0 80.9
atpE_p5 TCCGTGGAGTAAGACATCGA ATCTTCCGGTTCGTACAAGC 50.0 50.0 57.9 58.0 1607-1615 bp 1.0 80.5