Hotspot

ccsA-ndhD

SSC · 115265-115491 · 227 bp

Back to Acorus

Pi 0.0323
MarkerSeek score 63.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
227
variable_sites
12
pi
0.0323
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ACATAGTTATGGTTCATTCACA TCAGTTGACAAGGTGGAAGC 31.8 50.0 53.2 58.0 311-317 bp 1.0 43.3
ccsA-ndhD_p2 TGGAGTCAATCTATTAGGAATAGGT TCAGTTGACAAGGTGGAAGC 36.0 50.0 56.6 58.0 338-344 bp 1.0 43.0
ccsA-ndhD_p3 TGGAGTCAATCTATTAGGAATAGGTT TCAGTTGACAAGGTGGAAGC 34.6 50.0 57.2 58.0 338-344 bp 1.0 43.0
ccsA-ndhD_p4 TTGGAGTCAATCTATTAGGAATAGGT TCAGTTGACAAGGTGGAAGC 34.6 50.0 57.2 58.0 339-345 bp 1.0 43.0
ccsA-ndhD_p5 TGGAGTCAATCTATTAGGAATAGG TCAGTTGACAAGGTGGAAGC 37.5 50.0 55.0 58.0 338-344 bp 1.0 43.0