Hotspot

rpl32-tRNA-Leu (UAG)

SSC · 113275-114137 · 863 bp

Back to Acorus

Pi 0.0145
MarkerSeek score 61.10
Primer available yes
Label rpl32-tRNA-Leu (UAG)

Feature Metadata

label_name
rpl32-tRNA-Leu (UAG)
feature_type
igs
region
SSC
length_bp
863
variable_sites
20
pi
0.0145
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-tRNA-Leu (UAG)_p1 TCCACCGGACAGTCAAAAAG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 962-973 bp 1.0 82.0
rpl32-tRNA-Leu (UAG)_p2 TCCACCGGACAGTCAAAAAG AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 955-966 bp 1.0 81.5
rpl32-tRNA-Leu (UAG)_p3 TCCACCGGACAGTCAAAAAG CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.0 57.8 961-972 bp 1.0 81.4
rpl32-tRNA-Leu (UAG)_p4 TCCACCGGACAGTCAAAAAG CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 960-971 bp 1.0 81.4
rpl32-tRNA-Leu (UAG)_p5 AATCAGTTTCCACCGGACAG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.8 57.9 970-981 bp 1.0 80.8