Hotspot

petA-psbJ

LSC · 62840-63717 · 878 bp

Back to Acorus

Pi 0.0192
MarkerSeek score 57.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
878
variable_sites
30
pi
0.0192
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CAGGGCGATGCAGAAATAGT AACTGGCCGTTTTGATCCTT 50.0 45.0 58.0 58.0 1605-1616 bp 1.0 81.4
petA-psbJ_p2 CAGGGCGATGCAGAAATAGT ACTGGCCGTTTTGATCCTTT 50.0 45.0 58.0 58.0 1604-1615 bp 1.0 81.4
petA-psbJ_p3 GCATCGGATGGACATCAAGT AACTGGCCGTTTTGATCCTT 50.0 45.0 58.0 58.0 1728-1739 bp 1.0 81.4
petA-psbJ_p4 GCATCGGATGGACATCAAGT ACTGGCCGTTTTGATCCTTT 50.0 45.0 58.0 58.0 1727-1738 bp 1.0 81.4
petA-psbJ_p5 TGCATCGGATGGACATCAAG ACTGGCCGTTTTGATCCTTT 50.0 45.0 58.0 58.0 1728-1739 bp 1.0 81.4