Hotspot

tRNA-Met (CAU)-atpE

LSC · 53835-53999 · 165 bp

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Pi 0.0434
MarkerSeek score 58.10
Primer available yes
Label tRNA-Met (CAU)-atpE

Feature Metadata

label_name
tRNA-Met (CAU)-atpE
feature_type
igs
region
LSC
length_bp
165
variable_sites
11
pi
0.0434
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Met (CAU)-atpE_p1 CTTTGACGTAGGTCTGCCTC GAAATGATGCCGAGATGGGT 55.0 50.0 58.0 58.0 1003-1011 bp 1.0 81.8
tRNA-Met (CAU)-atpE_p2 CTTTGACGTAGGTCTGCCTC ACCGCGAAGGCTATGAATTT 55.0 45.0 58.0 58.0 1279-1287 bp 1.0 81.6
tRNA-Met (CAU)-atpE_p3 GCTCCATGTGCTCTGATTCA GAAATGATGCCGAGATGGGT 50.0 50.0 58.0 58.0 1075-1083 bp 1.0 81.4
tRNA-Met (CAU)-atpE_p4 GCTCCATGTGCTCTGATTCA ACCGCGAAGGCTATGAATTT 50.0 45.0 58.0 58.0 1351-1359 bp 1.0 81.3
tRNA-Met (CAU)-atpE_p5 CTTTGACGTAGGTCTGCCTC GCCACAGCTGTAGATATCGG 55.0 55.0 58.0 57.9 1115-1123 bp 1.0 81.3