Hotspot

ndhC-tRNA-Val (UAC)

LSC · 50987-52898 · 1912 bp

Back to Acorus

Pi 0.0109
MarkerSeek score 49.30
Primer available yes
Label ndhC-tRNA-Val (UAC)

Feature Metadata

label_name
ndhC-tRNA-Val (UAC)
feature_type
igs
region
LSC
length_bp
1912
variable_sites
35
pi
0.0109
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-tRNA-Val (UAC)_p1 TCATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 36.4 50.0 56.1 57.8 1538-1978 bp 1.0 52.8
ndhC-tRNA-Val (UAC)_p2 TCATATTCGTGAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 36.4 55.0 56.1 57.5 1537-1977 bp 1.0 50.1
ndhC-tRNA-Val (UAC)_p3 TCATATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 36.4 55.0 56.1 58.7 1526-1966 bp 1.0 49.0
ndhC-tRNA-Val (UAC)_p4 CATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 38.1 50.0 54.6 57.8 1537-1977 bp 1.0 48.8
ndhC-tRNA-Val (UAC)_p5 TCATATTCGTGAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 34.8 50.0 56.6 57.8 1538-1978 bp 1.0 48.8