Hotspot

ycf3-tRNA-Ser (GGA)

LSC · 44874-45701 · 828 bp

Back to Acorus

Pi 0.0127
MarkerSeek score 52.10
Primer available yes
Label ycf3-tRNA-Ser (GGA)

Feature Metadata

label_name
ycf3-tRNA-Ser (GGA)
feature_type
igs
region
LSC
length_bp
828
variable_sites
20
pi
0.0127
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-tRNA-Ser (GGA)_p1 TCTCCTGAAGTGGTCGGAAT CCCTCGGTAAACAAAAGCCT 50.0 50.0 58.1 58.1 983-991 bp 1.0 81.0
ycf3-tRNA-Ser (GGA)_p2 TCTCCTGAAGTGGTCGGAAT ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.1 58.2 1004-1012 bp 1.0 80.5
ycf3-tRNA-Ser (GGA)_p3 TCTCCTGAAGTGGTCGGAAT TTCCAATGCTACGCCTTCAA 50.0 45.0 58.1 57.8 954-962 bp 1.0 80.3
ycf3-tRNA-Ser (GGA)_p4 TCTCCTGAAGTGGTCGGAAT AGTTCCAATGCTACGCCTTC 50.0 50.0 58.1 58.3 956-964 bp 1.0 79.8
ycf3-tRNA-Ser (GGA)_p5 TTCTCCTGAAGTGGTCGGAA CCCTCGGTAAACAAAAGCCT 50.0 50.0 58.3 58.1 984-992 bp 1.0 79.2