Hotspot

psaA-ycf3

LSC · 42276-42893 · 618 bp

Back to Acorus

Pi 0.0113
MarkerSeek score 55.10
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
618
variable_sites
12
pi
0.0113
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GTTCTTGAAAAATGGCCGGG AGCGCAGAATTGGTTGAAGA 50.0 45.0 57.9 58.1 757-762 bp 1.0 80.3
psaA-ycf3_p2 GTTCTTGAAAAATGGCCGGG TGAAGATCACAAGGCGGTTC 50.0 50.0 57.9 58.2 743-748 bp 1.0 79.7
psaA-ycf3_p3 CCCATGCCTCGAAAGAAGTT AGCGCAGAATTGGTTGAAGA 50.0 45.0 58.2 58.1 732-737 bp 1.0 79.6
psaA-ycf3_p4 GTTCTTGAAAAATGGCCGGG TTGAAGATCACAAGGCGGTT 50.0 45.0 57.9 57.7 744-749 bp 1.0 79.0
psaA-ycf3_p5 CCCATGCCTCGAAAGAAGTT TGAAGATCACAAGGCGGTTC 50.0 50.0 58.2 58.2 718-723 bp 1.0 79.0