Hotspot

tRNA-Thr (GGU)-psbD

LSC · 31954-33318 · 1365 bp

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Pi 0.015
MarkerSeek score 64.50
Primer available yes
Label tRNA-Thr (GGU)-psbD

Feature Metadata

label_name
tRNA-Thr (GGU)-psbD
feature_type
igs
region
LSC
length_bp
1365
variable_sites
35
pi
0.015
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Thr (GGU)-psbD_p1 GTGGTAGAGTAACGCCATGG TCCGTAACCAGTCGTCCATA 55.0 50.0 58.1 57.9 1479-1528 bp 1.0 81.7
tRNA-Thr (GGU)-psbD_p2 TCAGTGGTAGAGTAACGCCA TCCGTAACCAGTCGTCCATA 50.0 50.0 58.1 57.9 1482-1531 bp 1.0 81.5
tRNA-Thr (GGU)-psbD_p3 GTGGTAGAGTAACGCCATGG GAAGAGCAATAGACCGGACC 55.0 55.0 58.1 57.8 1523-1572 bp 1.0 80.8
tRNA-Thr (GGU)-psbD_p4 GTGGTAGAGTAACGCCATGG GGAAGAGCAATAGACCGGAC 55.0 55.0 58.1 57.8 1524-1573 bp 1.0 80.8
tRNA-Thr (GGU)-psbD_p5 TCAGTGGTAGAGTAACGCCA GAAGAGCAATAGACCGGACC 50.0 55.0 58.1 57.8 1526-1575 bp 1.0 80.6