Hotspot

tRNA-Cys (GCA)-petN

LSC · 28157-29036 · 880 bp

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Pi 0.01
MarkerSeek score 68.60
Primer available yes
Label tRNA-Cys (GCA)-petN

Feature Metadata

label_name
tRNA-Cys (GCA)-petN
feature_type
igs
region
LSC
length_bp
880
variable_sites
14
pi
0.01
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Cys (GCA)-petN_p1 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.1 58.0 768-967 bp 1.0 84.5
tRNA-Cys (GCA)-petN_p2 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCAAGACTTACA 50.0 50.0 58.1 58.1 762-961 bp 1.0 83.8
tRNA-Cys (GCA)-petN_p3 GGGGGACTGCAAATCCTTTT TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.4 58.0 786-985 bp 1.0 82.3
tRNA-Cys (GCA)-petN_p4 TGTATTGTTTTGGCGGCATG TTAAAGCAGCCCAAGCAAGA 45.0 45.0 57.6 58.0 821-1020 bp 1.0 82.2
tRNA-Cys (GCA)-petN_p5 GTATTGTTTTGGCGGCATGG TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.4 58.0 820-1019 bp 1.0 82.2