Hotspot

tRNA-Lys (UUU)-rps16

LSC · 4288-4981 · 694 bp

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Pi 0.0108
MarkerSeek score 60.00
Primer available yes
Label tRNA-Lys (UUU)-rps16

Feature Metadata

label_name
tRNA-Lys (UUU)-rps16
feature_type
igs
region
LSC
length_bp
694
variable_sites
13
pi
0.0108
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Lys (UUU)-rps16_p1 AAAGCCGAGTACTCTACCGT TTTTCTGGAAAAGGGGGCTC 50.0 50.0 58.2 58.0 825-839 bp 1.0 80.1
tRNA-Lys (UUU)-rps16_p2 AAGCCGAGTACTCTACCGTT TTTTCTGGAAAAGGGGGCTC 50.0 50.0 58.2 58.0 824-838 bp 1.0 80.1
tRNA-Lys (UUU)-rps16_p3 CCGCACTTAAAAGCCGAGTA TTTTCTGGAAAAGGGGGCTC 50.0 50.0 58.3 58.0 834-848 bp 1.0 79.2
tRNA-Lys (UUU)-rps16_p4 AAAGCCGAGTACTCTACCGT GAAAAGGGGGCTCAACCTAC 50.0 55.0 58.2 58.2 818-832 bp 1.0 78.9
tRNA-Lys (UUU)-rps16_p5 AAGCCGAGTACTCTACCGTT GAAAAGGGGGCTCAACCTAC 50.0 55.0 58.2 58.2 817-831 bp 1.0 78.9