Hotspot

rps16

LSC · 4940-5880 · 941 bp

Back to Aconitum

Pi 0.0174

MarkerSeek score 62.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 941
variable_sites: 91
pi: 0.0174
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGGAAAGGAGGAGGGGAATA	TGTTTATGATGATTTGTGTTTCCT	55.0	29.2	57.8	55.3	938-1977 bp	0.792453	41.1
rps16_p2	GGGAAAGGAGGAGGGGAATA	TGTTTATGATGATTTGTGTTTCCTT	55.0	28.0	57.8	56.0	938-1977 bp	0.792453	41.1
rps16_p3	GGGAAAGGAGGAGGGGAATA	TGTTTATGATGATTTGTGTTTCC	55.0	30.4	57.8	53.9	938-1977 bp	0.792453	41.1
rps16_p4	GGGAAAGGAGGAGGGGAATA	ATGTTTATGATGATTTGTGTTTCCT	55.0	28.0	57.8	55.8	939-1978 bp	0.792453	41.1
rps16_p5	GGGAAAGGAGGAGGGGAATA	GTTTATGATGATTTGTGTTTCCT	55.0	30.4	57.8	53.6	937-1976 bp	0.792453	41.1