Hotspot

rpl20

LSC · 70115-70612 · 498 bp

Back to Aconitum

Pi 0.0387

MarkerSeek score 65.50

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 498
variable_sites: 64
pi: 0.0387
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	TATACTCGACCTTCCCGGAG	AGACCAGGAAAGGAGAAAAGA	55.0	42.9	57.7	56.2	461-778 bp	1.0	67.4
rpl20_p2	TATACTCGACCTTCCCGGAG	AGACCAGGAAAGGAGAAAAGAA	55.0	40.9	57.7	56.9	461-778 bp	1.0	65.3
rpl20_p3	TTATACTCGACCTTCCCGGA	AGACCAGGAAAGGAGAAAAGA	50.0	42.9	57.3	56.2	462-779 bp	1.0	63.6
rpl20_p4	TATACTCGACCTTCCCGGAG	ACCAGGAAAGGAGAAAAGAATGA	55.0	39.1	57.7	57.7	459-776 bp	1.0	63.3
rpl20_p5	TATACTCGACCTTCCCGGAG	GACCAGGAAAGGAGAAAAGA	55.0	45.0	57.7	54.6	460-777 bp	1.0	62.5