Hotspot

ndhE

SSC · 119348-119653 · 306 bp

Back to Aconitum

Pi 0.0036

MarkerSeek score 25.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 5
pi: 0.0036
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	AATTGGCGAATAGTCTATATTTT	CGAGATTGATGAGGAGTTGGT	26.1	47.6	52.0	57.5	363-369 bp	0.981132	40.8
ndhE_p2	ATTGGCGAATAGTCTATATTTTT	CGAGATTGATGAGGAGTTGGT	26.1	47.6	52.0	57.5	362-368 bp	0.981132	40.8
ndhE_p3	AATTGGCGAATAGTCTATATTTTT	CGAGATTGATGAGGAGTTGGT	25.0	47.6	52.9	57.5	363-369 bp	0.981132	40.8
ndhE_p4	AATTGGCGAATAGTCTATATTTT	TCGAGATTGATGAGGAGTTGG	26.1	47.6	52.0	57.2	364-370 bp	0.981132	40.8
ndhE_p5	ATTGGCGAATAGTCTATATTTTT	TCGAGATTGATGAGGAGTTGG	26.1	47.6	52.0	57.2	363-369 bp	0.981132	40.8