Hotspot

ndhE-ndhG

SSC · 119654-119791 · 138 bp

Back to Aconitum

Pi 0.0568

MarkerSeek score 68.30

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 138
variable_sites: 28
pi: 0.0568
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AAGACACATAAGCGCTCGAA	TGCTATGGCTCGTCAGTACT	45.0	50.0	57.6	58.2	238-269 bp	1.0	87.2
ndhE-ndhG_p2	AAGACACATAAGCGCTCGAA	GCCTTGATAGGTGCAATTGC	45.0	50.0	57.6	57.8	255-286 bp	0.981132	86.6
ndhE-ndhG_p3	AGACACATAAGCGCTCGAAC	GCCTTGATAGGTGCAATTGC	50.0	50.0	58.4	57.8	254-285 bp	0.981132	86.6
ndhE-ndhG_p4	CAAGACACATAAGCGCTCGA	GCCTTGATAGGTGCAATTGC	50.0	50.0	58.4	57.8	256-287 bp	0.981132	86.6
ndhE-ndhG_p5	TCAAGACACATAAGCGCTCG	GCCTTGATAGGTGCAATTGC	50.0	50.0	58.4	57.8	257-288 bp	0.981132	86.6