Hotspot

ccsA-ndhD

SSC · 116962-117233 · 272 bp

Back to Aconitum

Pi 0.0327

MarkerSeek score 61.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 272
variable_sites: 38
pi: 0.0327
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCTATGCAGCTCTTCTGTGT	CACTTGCATTGCCTGGAATG	50.0	50.0	58.0	58.0	942-1181 bp	1.0	89.1
ccsA-ndhD_p2	GCTATGCAGCTCTTCTGTGT	ACTTGCATTGCCTGGAATGA	50.0	45.0	58.0	58.1	941-1180 bp	1.0	88.5
ccsA-ndhD_p3	GCTATGCAGCTCTTCTGTGT	TCACTTGCATTGCCTGGAAT	50.0	45.0	58.0	58.1	943-1182 bp	1.0	88.5
ccsA-ndhD_p4	GCTATGCAGCTCTTCTGTGT	TTGCATTGCCTGGAATGAGT	50.0	45.0	58.0	58.1	939-1178 bp	1.0	88.5
ccsA-ndhD_p5	GCTATGCAGCTCTTCTGTGT	TCGGTCAGCGCAATCTAAAA	50.0	45.0	58.0	57.9	1209-1448 bp	1.0	87.9