Hotspot

ycf1-ndhF

SSC · 112509-112675 · 167 bp

Back to Aconitum

Pi 0.108

MarkerSeek score 63.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 167
variable_sites: 81
pi: 0.108
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 0.65
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AGAATGCCGTCGCCTATTTT	AGTGGAGGACGGATCTCTTC	45.0	55.0	58.0	58.2	564-909 bp	1.0	89.9
ycf1-ndhF_p2	AGAATGCCGTCGCCTATTTT	GTGGAGGACGGATCTCTTCT	45.0	55.0	58.0	58.2	563-908 bp	1.0	89.9
ycf1-ndhF_p3	ACAGGAACAAGAGGGATCCA	AGTGGAGGACGGATCTCTTC	50.0	55.0	58.0	58.2	466-811 bp	1.0	89.8
ycf1-ndhF_p4	ACAGGAACAAGAGGGATCCA	GTGGAGGACGGATCTCTTCT	50.0	55.0	58.0	58.2	465-810 bp	1.0	89.8
ycf1-ndhF_p5	AAGAGGGATCCACCGAAGAA	GTGGAGGACGGATCTCTTCT	50.0	55.0	58.0	58.2	457-802 bp	1.0	89.8