Hotspot

trnF-GAA-ndhJ

LSC · 50602-51285 · 684 bp

Back to Aconitum

Pi 0.0113

MarkerSeek score 61.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 46
pi: 0.0113
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TCCACGCCTGAAACGTATTT	50.0	45.0	58.1	57.8	808-830 bp	1.0	86.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	780-802 bp	1.0	86.4
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CATCCACGCCTGAAACGTAT	50.0	50.0	58.1	58.3	810-832 bp	1.0	85.4
trnF-GAA-ndhJ_p4	GTCGGGAAAAGTCGGGATAG	TCCACGCCTGAAACGTATTT	55.0	45.0	57.8	57.8	857-879 bp	1.0	85.1
trnF-GAA-ndhJ_p5	GGGAAAAGTCGGGATAGCTC	TCCACGCCTGAAACGTATTT	55.0	45.0	57.8	57.8	854-876 bp	1.0	84.9