Hotspot

trnT-GGU-psbD

LSC · 33244-34762 · 1519 bp

Back to Aconitum

Pi 0.00952

MarkerSeek score 65.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1519
variable_sites: 96
pi: 0.00952
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1352-1790 bp	1.0	85.8
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	GACCGGACCAACCTACAAAA	50.0	50.0	57.9	57.7	1366-1804 bp	1.0	85.6
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	CGCAACCAGTCATCCATAGT	50.0	50.0	57.9	57.7	1331-1769 bp	1.0	85.3
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	TAGACCGGACCAACCTACAA	50.0	50.0	57.9	57.7	1368-1806 bp	1.0	85.2
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	GAGCAATAGACCGGACCAAC	50.0	55.0	57.9	58.3	1374-1812 bp	1.0	84.9