Hotspot

rpoB-trnC-GCA

LSC · 26956-28159 · 1204 bp

Back to Aconitum

Pi 0.0112

MarkerSeek score 62.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1204
variable_sites: 79
pi: 0.0112
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCTCATTTCCATCCCG	CTTGATCAGACGACACCCAG	50.0	55.0	57.3	58.0	1270-1308 bp	1.0	83.6
rpoB-trnC-GCA_p2	GTTCTTCCGTCAATCCCTGA	CTTGATCAGACGACACCCAG	50.0	55.0	57.2	58.0	1347-1385 bp	1.0	82.5
rpoB-trnC-GCA_p3	AGTTCTTCCGTCAATCCCTG	CTTGATCAGACGACACCCAG	50.0	55.0	57.2	58.0	1348-1386 bp	1.0	82.5
rpoB-trnC-GCA_p4	ATTCCCTCATTTCCATCCCG	TGATCAGACGACACCCAGAT	50.0	50.0	57.3	58.1	1268-1306 bp	1.0	82.5
rpoB-trnC-GCA_p5	ATTCCCTCATTTCCATCCCG	CAAGCAAGGGGATTTCGAGT	50.0	50.0	57.3	58.2	1295-1333 bp	1.0	82.2