Hotspot

trnH-GUG-psbA

LSC · 197-430 · 234 bp

Back to Aconitum

Pi 0.0302

MarkerSeek score 61.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 28
pi: 0.0302
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	299-374 bp	0.981132	88.8
trnH-GUG-psbA_p2	AAATAGGATGGGCGAACGAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.1	58.0	355-430 bp	0.962264	87.8
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.2	58.0	300-375 bp	0.962264	87.6
trnH-GUG-psbA_p4	AAATAGGATGGGCGAACGAC	TATCATCAACCGCGCTAACC	50.0	50.0	58.1	58.1	366-441 bp	0.962264	86.8
trnH-GUG-psbA_p5	AAATAGGATGGGCGAACGAC	CAGATATCATCAACCGCGCT	50.0	50.0	58.1	58.2	370-445 bp	0.962264	86.2