Hotspot

ycf1

SSC · 121597-126978 · 5382 bp

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Pi 0.02

MarkerSeek score 55.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5382
variable_sites: 158
pi: 0.02
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAATTTCACTCCTTTCTTTAAT	AGCTATTCGATCTAATAAGGG	26.1	38.1	52.3	52.0	165 bp	1.0	46.8
ycf1_p2	TCAATTTCACTCCTTTCTTTAATT	AGCTATTCGATCTAATAAGGG	25.0	38.1	53.1	52.0	165 bp	1.0	46.8
ycf1_p3	TCAATTTCACTCCTTTCTTTAAT	GTAGCTATTCGATCTAATAAGGG	26.1	39.1	52.3	53.7	167 bp	1.0	46.7
ycf1_p4	TCAATTTCACTCCTTTCTTTAATT	GTAGCTATTCGATCTAATAAGGG	25.0	39.1	53.1	53.7	167 bp	1.0	46.7
ycf1_p5	TCAATTTCACTCCTTTCTTTAAT	TAGCTATTCGATCTAATAAGGG	26.1	36.4	52.3	52.2	166 bp	1.0	46.7