Hotspot

rps15-ycf1

SSC · 121223-121596 · 374 bp

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Pi 0.0339

MarkerSeek score 65.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 374
variable_sites: 19
pi: 0.0339
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACTGATCCGGGGTTTTC	TCCACGGTTGAAAATACGGT	50.0	45.0	57.8	57.5	463-476 bp	1.0	77.8
rps15-ycf1_p2	CAACTGATCCGGGGTTTTCT	TCCACGGTTGAAAATACGGT	50.0	45.0	57.8	57.5	462-475 bp	1.0	77.8
rps15-ycf1_p3	ACTGATCCGGGGTTTTCTTC	TCCACGGTTGAAAATACGGT	50.0	45.0	57.5	57.5	460-473 bp	1.0	75.5
rps15-ycf1_p4	TTCAACTGATCCGGGGTTTT	TCCACGGTTGAAAATACGGT	45.0	45.0	57.3	57.5	464-477 bp	1.0	74.0
rps15-ycf1_p5	TCAACTGATCCGGGGTTTTC	ATCCACGGTTGAAAATACGGT	50.0	42.9	57.8	57.9	464-477 bp	1.0	73.7