Hotspot

ndhA-ndhH

SSC · 119663-119663 · 1 bp

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Pi 0.667

MarkerSeek score 65.10

Primer available yes

Label ndhA-ndhH

Feature Metadata

label_name: ndhA-ndhH
feature_type: igs
region: SSC
length_bp: 1
variable_sites: 1
pi: 0.667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-ndhH_p1	TCTTGGACTTCTGTTGTATCA	CGTCCACCGGGTTTTATCAA	38.1	50.0	54.2	58.2	150 bp	1.0	40.8
ndhA-ndhH_p2	TCTTGGACTTCTGTTGTATCAA	CGTCCACCGGGTTTTATCAA	36.4	50.0	55.0	58.2	150 bp	1.0	40.5
ndhA-ndhH_p3	ATCTTGGACTTCTGTTGTATCA	CGTCCACCGGGTTTTATCAA	36.4	50.0	54.8	58.2	151 bp	1.0	40.5
ndhA-ndhH_p4	TGATATCTTGGACTTCTGTTGT	CGTCCACCGGGTTTTATCAA	36.4	50.0	54.8	58.2	155 bp	1.0	40.5
ndhA-ndhH_p5	TCTTGGACTTCTGTTGTATCAAT	CGTCCACCGGGTTTTATCAA	34.8	50.0	55.6	58.2	150 bp	1.0	40.5