Hotspot

ndhG-ndhI

SSC · 116388-116741 · 354 bp

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Pi 0.0265

MarkerSeek score 70.60

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 354
variable_sites: 14
pi: 0.0265
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GTAGCGTTTTGGGCAAATCC	AGGCCGTTTACCAATGTCAG	50.0	50.0	58.0	58.2	435-458 bp	1.0	81.4
ndhG-ndhI_p2	GTAGCGTTTTGGGCAAATCC	TGCTTTAGGCCGTTTACCAA	50.0	45.0	58.0	57.7	441-464 bp	1.0	80.6
ndhG-ndhI_p3	GTAGCGTTTTGGGCAAATCC	TTGCTTTAGGCCGTTTACCA	50.0	45.0	58.0	57.7	442-465 bp	1.0	80.6
ndhG-ndhI_p4	ATCATGTAGCGTTTTGGGCA	AGGCCGTTTACCAATGTCAG	45.0	50.0	58.2	58.2	440-463 bp	1.0	80.1
ndhG-ndhI_p5	TACAAGACCCGATCCCAGAA	AGGCCGTTTACCAATGTCAG	50.0	50.0	57.8	58.2	473-496 bp	1.0	79.3