Hotspot

ndhD-psaC

SSC · 114626-114753 · 128 bp

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Pi 0.0312

MarkerSeek score 64.30

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 128
variable_sites: 6
pi: 0.0312
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AAATTAAGGAACCGCCAGCT	GCTTGTCCGACGGATTTCTT	45.0	50.0	57.8	58.6	270-281 bp	1.0	75.5
ndhD-psaC_p2	TAAGGAACCGCCAGCTATTG	GCTTGTCCGACGGATTTCTT	50.0	50.0	57.7	58.6	266-277 bp	1.0	74.7
ndhD-psaC_p3	AAATTAAGGAACCGCCAGCT	TGTTGGTTGTAAAAGGTGCG	45.0	45.0	57.8	57.1	295-306 bp	1.0	73.0
ndhD-psaC_p4	TAAGGAACCGCCAGCTATTG	TGTTGGTTGTAAAAGGTGCG	50.0	45.0	57.7	57.1	291-302 bp	1.0	72.2
ndhD-psaC_p5	AAATTAAGGAACCGCCAGCT	GCTTGTCCGACGGATTTCT	45.0	52.6	57.8	57.9	270-281 bp	1.0	70.9