Hotspot

petA-psbJ

LSC · 62451-63202 · 752 bp

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Pi 0.0191

MarkerSeek score 71.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 21
pi: 0.0191
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGATCATCC	50.0	50.0	57.8	57.1	889-958 bp	1.0	73.4
petA-psbJ_p2	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGATCATCC	55.0	50.0	58.3	57.1	894-963 bp	1.0	72.9
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	ATTCCGGATTGGGATCATCC	55.0	50.0	58.4	57.1	898-967 bp	1.0	72.4
petA-psbJ_p4	CGTGTCCAAGGTCTCTTGTT	TGGTTCATATTCCGGATTGGG	50.0	47.6	57.8	57.8	897-966 bp	1.0	70.7
petA-psbJ_p5	CGTTACGTGTCCAAGGTCTC	TGGTTCATATTCCGGATTGGG	55.0	47.6	58.3	57.8	902-971 bp	1.0	70.2