Hotspot

psbK-trnQ-UUG

LSC · 54894-55455 · 562 bp

Back to Acmispon

Pi 0.0227

MarkerSeek score 68.90

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 562
variable_sites: 18
pi: 0.0227
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	CCTCGGGCAATTTATCCAAGA	TATATGTGCTCTGGGACGGA	47.6	50.0	58.1	57.6	643-729 bp	1.0	74.5
psbK-trnQ-UUG_p2	CCTCGGGCAATTTATCCAAGA	ATATGTGCTCTGGGACGGAA	47.6	50.0	58.1	58.5	642-728 bp	1.0	73.5
psbK-trnQ-UUG_p3	CCTCGGGCAATTTATCCAAGA	GACCAAAACCCGTTGCCTTA	47.6	50.0	58.1	58.7	599-685 bp	1.0	72.2
psbK-trnQ-UUG_p4	CCTCGGGCAATTTATCCAAG	TATATGTGCTCTGGGACGGA	50.0	50.0	56.5	57.6	643-729 bp	1.0	71.5
psbK-trnQ-UUG_p5	CCTCGGGCAATTTATCCAAG	ATATGTGCTCTGGGACGGAA	50.0	50.0	56.5	58.5	642-728 bp	1.0	70.4