Hotspot

trnE-UUC-trnY-GUA

LSC · 30627-30686 · 60 bp

Back to Acmispon

Pi 0.0333

MarkerSeek score 65.50

Primer available yes

Label trnE-UUC-trnY-GUA

Feature Metadata

label_name: trnE-UUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 3
pi: 0.0333
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnY-GUA_p1	TGAACGTAGTACAAAGGCGG	TGGATTTGAACCAGCGTAGG	50.0	50.0	57.9	57.9	239 bp	1.0	79.6
trnE-UUC-trnY-GUA_p2	AACGTAGTACAAAGGCGGTC	TGGATTTGAACCAGCGTAGG	50.0	50.0	57.9	57.9	237 bp	1.0	79.6
trnE-UUC-trnY-GUA_p3	GAACGTAGTACAAAGGCGGT	TGGATTTGAACCAGCGTAGG	50.0	50.0	57.9	57.9	238 bp	1.0	79.6
trnE-UUC-trnY-GUA_p4	AAGTATGCCCCCATCGTCTA	TGGATTTGAACCAGCGTAGG	50.0	50.0	57.9	57.9	213 bp	1.0	79.5
trnE-UUC-trnY-GUA_p5	TCTCTTTCAAGGAGGCAACG	TGGATTTGAACCAGCGTAGG	50.0	50.0	57.8	57.9	178 bp	1.0	79.1