Hotspot

rbcL-atpB

LSC · 6520-7322 · 803 bp

Back to Acmispon

Pi 0.00998

MarkerSeek score 65.20

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 12
pi: 0.00998
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	AACACCAGCTTTGAACCCAA	TACATCTAGCACCGGACCAA	45.0	50.0	58.1	58.1	953-958 bp	1.0	78.8
rbcL-atpB_p2	AACACCAGCTTTGAACCCAA	TTACATCTAGCACCGGACCA	45.0	50.0	58.1	58.1	954-959 bp	1.0	78.8
rbcL-atpB_p3	AACACCAGCTTTGAACCCAA	GTTACATCTAGCACCGGACC	45.0	55.0	58.1	57.8	955-960 bp	1.0	78.2
rbcL-atpB_p4	AACACCAGCTTTGAACCCAA	CATCTAGCACCGGACCAATT	45.0	50.0	58.1	57.7	951-956 bp	1.0	77.3
rbcL-atpB_p5	GCTTTGAACCCAACACTTGC	TACATCTAGCACCGGACCAA	50.0	50.0	58.4	58.1	946-951 bp	1.0	76.5