primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	131	377	309-391	1.000000	85.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	131	377	323-405	1.000000	84.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	131	377	322-404	1.000000	84.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	131	377	324-406	1.000000	84.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CGGTCGAGGCTCCATCTATA	20	20	55.0	55.0	58.1	58.2	0.0	9.3	0.306414	131	377	290-372	1.000000	84.8
trnK-UUU-rbcL_p1	trnK-UUU-rbcL	1	AAAGCCGAGTACTCTACCGT	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	58.2	58.0	20.3	13.4	0.203950	4389	5091	790-812	1.000000	83.9
trnK-UUU-rbcL_p2	trnK-UUU-rbcL	2	AAGCCGAGTACTCTACCGTT	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	58.2	58.0	20.3	13.4	0.203950	4389	5091	789-811	1.000000	83.9
trnK-UUU-rbcL_p3	trnK-UUU-rbcL	3	TACCGTTGAGTTAGCAACCC	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	57.5	58.0	0.0	13.4	0.496287	4389	5091	776-798	1.000000	81.6
trnK-UUU-rbcL_p4	trnK-UUU-rbcL	4	AAAGCCGAGTACTCTACCGT	GCTGCTGCTTGTGAAGTATG	20	20	50.0	50.0	58.2	57.5	20.3	8.5	0.707766	4389	5091	778-800	1.000000	79.9
trnK-UUU-rbcL_p5	trnK-UUU-rbcL	5	AAGCCGAGTACTCTACCGTT	GCTGCTGCTTGTGAAGTATG	20	20	50.0	50.0	58.2	57.5	20.3	8.5	0.707766	4389	5091	777-799	1.000000	79.9
rbcL-atpB_p1	rbcL-atpB	1	AACACCAGCTTTGAACCCAA	TACATCTAGCACCGGACCAA	20	20	45.0	50.0	58.1	58.1	0.0	9.5	0.285403	6520	7322	953-958	1.000000	78.8
rbcL-atpB_p2	rbcL-atpB	2	AACACCAGCTTTGAACCCAA	TTACATCTAGCACCGGACCA	20	20	45.0	50.0	58.1	58.1	0.0	9.5	0.285403	6520	7322	954-959	1.000000	78.8
rbcL-atpB_p3	rbcL-atpB	3	AACACCAGCTTTGAACCCAA	GTTACATCTAGCACCGGACC	20	20	45.0	55.0	58.1	57.8	0.0	9.5	0.361177	6520	7322	955-960	1.000000	78.2
rbcL-atpB_p4	rbcL-atpB	4	AACACCAGCTTTGAACCCAA	CATCTAGCACCGGACCAATT	20	20	45.0	50.0	58.1	57.7	0.0	9.5	0.472761	6520	7322	951-956	1.000000	77.3
rbcL-atpB_p5	rbcL-atpB	5	GCTTTGAACCCAACACTTGC	TACATCTAGCACCGGACCAA	20	20	50.0	50.0	58.4	58.1	0.0	9.5	0.573892	6520	7322	946-951	1.000000	76.5
atpE-trnM-CAU_p1	atpE-trnM-CAU	1	CAGACGAGCTAGAACACGAG	TATTGCTTTCATACGGCGGG	20	20	55.0	50.0	57.8	58.4	0.0	13.2	0.578067	9218	9365	239-243	1.000000	80.4
atpE-trnM-CAU_p2	atpE-trnM-CAU	2	CTCAGACGAGCTAGAACACG	TATTGCTTTCATACGGCGGG	20	20	55.0	50.0	57.8	58.4	0.0	13.2	0.578067	9218	9365	241-245	1.000000	80.3
atpE-trnM-CAU_p3	atpE-trnM-CAU	3	AGACGAGCTAGAACACGAGT	TATTGCTTTCATACGGCGGG	20	20	50.0	50.0	58.2	58.4	0.0	13.2	0.600367	9218	9365	238-242	1.000000	80.2
atpE-trnM-CAU_p4	atpE-trnM-CAU	4	TCAGACGAGCTAGAACACGA	TATTGCTTTCATACGGCGGG	20	20	50.0	50.0	58.2	58.4	0.0	13.2	0.606725	9218	9365	240-244	1.000000	80.1
atpE-trnM-CAU_p5	atpE-trnM-CAU	5	GGCTAAGGGCAAGAGACAAA	TATTGCTTTCATACGGCGGG	20	20	50.0	50.0	57.8	58.4	0.0	13.2	0.611699	9218	9365	281-285	1.000000	79.4
trnT-UGU-rps4_p1	trnT-UGU-rps4	1	TGATATTCCTGTGCCCATGC	ATGGAGCGCGATTGATTTCT	20	20	50.0	45.0	58.0	58.0	0.0	12.1	0.055485	14788	15107	543-551	1.000000	82.0
trnT-UGU-rps4_p2	trnT-UGU-rps4	2	CTCAGAGGTTAGAGCATCGC	ATGGAGCGCGATTGATTTCT	20	20	55.0	45.0	57.9	58.0	0.0	12.1	0.173698	14788	15107	491-499	1.000000	81.4
trnT-UGU-rps4_p3	trnT-UGU-rps4	3	ATGGTCATCGGTTCGATTCC	ATGGAGCGCGATTGATTTCT	20	20	50.0	45.0	57.8	58.0	0.0	12.1	0.281452	14788	15107	459-467	1.000000	80.7
trnT-UGU-rps4_p4	trnT-UGU-rps4	4	TGATATTCCTGTGCCCATGC	CCCAGACGGCGTATCTTTTT	20	20	50.0	50.0	58.0	58.3	0.0	14.7	0.280364	14788	15107	474-482	1.000000	80.6
trnT-UGU-rps4_p5	trnT-UGU-rps4	5	TGATATTCCTGTGCCCATGC	AAGTTCGCTTCCGACTGTAG	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.363824	14788	15107	523-531	1.000000	79.7
trnE-UUC-trnY-GUA_p1	trnE-UUC-trnY-GUA	1	TGAACGTAGTACAAAGGCGG	TGGATTTGAACCAGCGTAGG	20	20	50.0	50.0	57.9	57.9	0.4	0.0	0.176787	30627	30686	239-239	1.000000	79.6
trnE-UUC-trnY-GUA_p2	trnE-UUC-trnY-GUA	2	AACGTAGTACAAAGGCGGTC	TGGATTTGAACCAGCGTAGG	20	20	50.0	50.0	57.9	57.9	0.4	0.0	0.178212	30627	30686	237-237	1.000000	79.6
trnE-UUC-trnY-GUA_p3	trnE-UUC-trnY-GUA	3	GAACGTAGTACAAAGGCGGT	TGGATTTGAACCAGCGTAGG	20	20	50.0	50.0	57.9	57.9	0.4	0.0	0.178212	30627	30686	238-238	1.000000	79.6
trnE-UUC-trnY-GUA_p4	trnE-UUC-trnY-GUA	4	AAGTATGCCCCCATCGTCTA	TGGATTTGAACCAGCGTAGG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.205060	30627	30686	213-213	1.000000	79.5
trnE-UUC-trnY-GUA_p5	trnE-UUC-trnY-GUA	5	TCTCTTTCAAGGAGGCAACG	TGGATTTGAACCAGCGTAGG	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.277782	30627	30686	178-178	1.000000	79.1
psbM-petN_p1	psbM-petN	1	GCTACTGCGCTCTTCATTCT	TGAAATGGAGGGGGCAAAAA	20	20	50.0	45.0	58.1	57.9	9.7	0.0	0.175983	31841	32665	1406-1433	1.000000	83.4
psbM-petN_p2	psbM-petN	2	TTACAGTGCAACGGTTGGAA	TGAAATGGAGGGGGCAAAAA	20	20	45.0	45.0	58.0	57.9	21.4	0.0	0.149047	31841	32665	2011-2036	1.000000	83.0
psbM-petN_p3	psbM-petN	3	TTACAGTGCAACGGTTGGAA	TAGTAAGTCTTGCCTGGGCT	20	20	45.0	50.0	58.0	58.0	21.4	13.1	0.087197	31841	32665	1594-1607	1.000000	82.5
psbM-petN_p4	psbM-petN	4	GCTACTGCGCTCTTCATTCT	TAGTAAGTCTTGCCTGGGCT	20	20	50.0	50.0	58.1	58.0	9.7	13.1	0.114132	31841	32665	989-1005	1.000000	82.5
psbM-petN_p5	psbM-petN	5	TTGCTACTGCGCTCTTCATT	TAGTAAGTCTTGCCTGGGCT	20	20	45.0	50.0	57.9	58.0	9.7	13.1	0.146520	31841	32665	991-1007	1.000000	82.2
trnR-UCU-trnG-UCC_p1	trnR-UCU-trnG-UCC	1	TGCGCCCAATAGGATTTGAA	AGCCTTCCAAGCTAACGATG	20	20	45.0	50.0	57.9	58.0	4.2	0.0	0.166137	52092	52297	322-372	1.000000	84.2
trnR-UCU-trnG-UCC_p2	trnR-UCU-trnG-UCC	2	TTGCGCCCAATAGGATTTGA	AGCCTTCCAAGCTAACGATG	20	20	45.0	50.0	57.9	58.0	4.2	0.0	0.166137	52092	52297	323-373	1.000000	84.2
trnR-UCU-trnG-UCC_p3	trnR-UCU-trnG-UCC	3	TGCGCCCAATAGGATTTGAA	ACCCTTAGCCTTCCAAGCTA	20	20	45.0	50.0	57.9	58.0	4.2	0.0	0.169453	52092	52297	328-378	1.000000	84.1
trnR-UCU-trnG-UCC_p4	trnR-UCU-trnG-UCC	4	TTGCGCCCAATAGGATTTGA	ACCCTTAGCCTTCCAAGCTA	20	20	45.0	50.0	57.9	58.0	4.2	0.0	0.169453	52092	52297	329-379	1.000000	84.0
trnR-UCU-trnG-UCC_p5	trnR-UCU-trnG-UCC	5	GCGCCCAATAGGATTTGAAC	AGCCTTCCAAGCTAACGATG	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.521858	52092	52297	321-371	1.000000	81.3
trnG-UCC-trnS-GCU_p1	trnG-UCC-trnS-GCU	1	ACGAATCGCACTTTTACCACT	CAATCCGACGCTTTAGTCCA	21	20	42.9	50.0	58.2	58.0	1.2	0.0	1.241837	53094	53896	872-885	1.000000	72.5
trnG-UCC-trnS-GCU_p2	trnG-UCC-trnS-GCU	2	ACGAATCGCACTTTTACCACT	AATCCGACGCTTTAGTCCAC	21	20	42.9	50.0	58.2	58.0	1.2	0.0	1.243225	53094	53896	871-884	1.000000	72.5
trnG-UCC-trnS-GCU_p3	trnG-UCC-trnS-GCU	3	ACGAATCGCACTTTTACCAC	CAATCCGACGCTTTAGTCCA	20	20	45.0	50.0	56.8	58.0	1.2	0.0	1.248863	53094	53896	872-885	1.000000	72.5
trnG-UCC-trnS-GCU_p4	trnG-UCC-trnS-GCU	4	ACGAATCGCACTTTTACCAC	AATCCGACGCTTTAGTCCAC	20	20	45.0	50.0	56.8	58.0	1.2	0.0	1.250251	53094	53896	871-884	1.000000	72.5
trnG-UCC-trnS-GCU_p5	trnG-UCC-trnS-GCU	5	TCCATAGAACGAATCGCACT	CAATCCGACGCTTTAGTCCA	20	20	45.0	50.0	56.8	58.0	1.2	0.0	1.253085	53094	53896	880-893	1.000000	72.4
psbK-trnQ-UUG_p1	psbK-trnQ-UUG	1	CCTCGGGCAATTTATCCAAGA	TATATGTGCTCTGGGACGGA	21	20	47.6	50.0	58.1	57.6	0.0	0.0	1.444064	54894	55455	643-729	1.000000	74.5
psbK-trnQ-UUG_p2	psbK-trnQ-UUG	2	CCTCGGGCAATTTATCCAAGA	ATATGTGCTCTGGGACGGAA	21	20	47.6	50.0	58.1	58.5	0.0	0.0	1.572990	54894	55455	642-728	1.000000	73.5
psbK-trnQ-UUG_p3	psbK-trnQ-UUG	3	CCTCGGGCAATTTATCCAAGA	GACCAAAACCCGTTGCCTTA	21	20	47.6	50.0	58.1	58.7	0.0	0.0	1.748543	54894	55455	599-685	1.000000	72.2
psbK-trnQ-UUG_p4	psbK-trnQ-UUG	4	CCTCGGGCAATTTATCCAAG	TATATGTGCTCTGGGACGGA	20	20	50.0	50.0	56.5	57.6	0.0	0.0	1.826060	54894	55455	643-729	1.000000	71.5
psbK-trnQ-UUG_p5	psbK-trnQ-UUG	5	CCTCGGGCAATTTATCCAAG	ATATGTGCTCTGGGACGGAA	20	20	50.0	50.0	56.5	58.5	0.0	0.0	1.954986	54894	55455	642-728	1.000000	70.4
petA-psbJ_p1	petA-psbJ	1	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGATCATCC	20	20	50.0	50.0	57.8	57.1	5.6	4.1	1.127644	62451	63202	889-958	1.000000	73.4
petA-psbJ_p2	petA-psbJ	2	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGATCATCC	20	20	55.0	50.0	58.3	57.1	0.0	4.1	1.192512	62451	63202	894-963	1.000000	72.9
petA-psbJ_p3	petA-psbJ	3	GATCCGTTACGTGTCCAAGG	ATTCCGGATTGGGATCATCC	20	20	55.0	50.0	58.4	57.1	3.5	4.1	1.250163	62451	63202	898-967	1.000000	72.4
petA-psbJ_p4	petA-psbJ	4	CGTGTCCAAGGTCTCTTGTT	TGGTTCATATTCCGGATTGGG	20	21	50.0	47.6	57.8	57.8	5.6	4.1	1.462666	62451	63202	897-966	1.000000	70.7
petA-psbJ_p5	petA-psbJ	5	CGTTACGTGTCCAAGGTCTC	TGGTTCATATTCCGGATTGGG	20	21	55.0	47.6	58.3	57.8	0.0	4.1	1.527533	62451	63202	902-971	1.000000	70.2
rpl16_p1	rpl16	1	ACCGTTGCTACGTTTGAGTT	GCAGAATTCATAGCCGGACA	20	20	45.0	50.0	58.1	58.0	18.9	5.0	0.102410	80305	81784	2230-2237	1.000000	81.1
rpl16_p2	rpl16	2	ACCGTTGCTACGTTTGAGTT	TGCAGAATTCATAGCCGGAC	20	20	45.0	50.0	58.1	58.0	18.9	5.0	0.102410	80305	81784	2231-2238	1.000000	81.1
rpl16_p3	rpl16	3	GCTCCGCTATTATCGGCTAC	GCAGAATTCATAGCCGGACA	20	20	55.0	50.0	58.1	58.0	1.1	5.0	0.135753	80305	81784	2055-2062	1.000000	81.0
rpl16_p4	rpl16	4	GCTCCGCTATTATCGGCTAC	TGCAGAATTCATAGCCGGAC	20	20	55.0	50.0	58.1	58.0	1.1	5.0	0.135753	80305	81784	2056-2063	1.000000	81.0
rpl16_p5	rpl16	5	ACCGTTGCTACGTTTGAGTT	TCGAGGGAATTGGACGGATA	20	20	45.0	50.0	58.1	57.9	18.9	0.0	0.132175	80305	81784	2442-2449	1.000000	80.8
ndhD-psaC_p1	ndhD-psaC	1	AAATTAAGGAACCGCCAGCT	GCTTGTCCGACGGATTTCTT	20	20	45.0	50.0	57.8	58.6	0.0	21.5	0.776263	114626	114753	270-281	1.000000	75.5
ndhD-psaC_p2	ndhD-psaC	2	TAAGGAACCGCCAGCTATTG	GCTTGTCCGACGGATTTCTT	20	20	50.0	50.0	57.7	58.6	0.0	21.5	0.885904	114626	114753	266-277	1.000000	74.7
ndhD-psaC_p3	ndhD-psaC	3	AAATTAAGGAACCGCCAGCT	TGTTGGTTGTAAAAGGTGCG	20	20	45.0	45.0	57.8	57.1	0.0	0.0	1.069673	114626	114753	295-306	1.000000	73.0
ndhD-psaC_p4	ndhD-psaC	4	TAAGGAACCGCCAGCTATTG	TGTTGGTTGTAAAAGGTGCG	20	20	50.0	45.0	57.7	57.1	0.0	0.0	1.179314	114626	114753	291-302	1.000000	72.2
ndhD-psaC_p5	ndhD-psaC	5	AAATTAAGGAACCGCCAGCT	GCTTGTCCGACGGATTTCT	20	19	45.0	52.6	57.8	57.9	0.0	21.5	1.354971	114626	114753	270-281	1.000000	70.9
ndhG-ndhI_p1	ndhG-ndhI	1	GTAGCGTTTTGGGCAAATCC	AGGCCGTTTACCAATGTCAG	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.187984	116388	116741	435-458	1.000000	81.4
ndhG-ndhI_p2	ndhG-ndhI	2	GTAGCGTTTTGGGCAAATCC	TGCTTTAGGCCGTTTACCAA	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.282739	116388	116741	441-464	1.000000	80.6
ndhG-ndhI_p3	ndhG-ndhI	3	GTAGCGTTTTGGGCAAATCC	TTGCTTTAGGCCGTTTACCA	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.282739	116388	116741	442-465	1.000000	80.6
ndhG-ndhI_p4	ndhG-ndhI	4	ATCATGTAGCGTTTTGGGCA	AGGCCGTTTACCAATGTCAG	20	20	45.0	50.0	58.2	58.2	0.0	0.0	0.345900	116388	116741	440-463	1.000000	80.1
ndhG-ndhI_p5	ndhG-ndhI	5	TACAAGACCCGATCCCAGAA	AGGCCGTTTACCAATGTCAG	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.420064	116388	116741	473-496	1.000000	79.3
ndhA-ndhH_p1	ndhA-ndhH	1	TCTTGGACTTCTGTTGTATCA	CGTCCACCGGGTTTTATCAA	21	20	38.1	50.0	54.2	58.2	0.0	6.3	4.967297	119663	119663	150-150	1.000000	40.8
ndhA-ndhH_p2	ndhA-ndhH	2	TCTTGGACTTCTGTTGTATCAA	CGTCCACCGGGTTTTATCAA	22	20	36.4	50.0	55.0	58.2	0.0	6.3	5.141966	119663	119663	150-150	1.000000	40.5
ndhA-ndhH_p3	ndhA-ndhH	3	ATCTTGGACTTCTGTTGTATCA	CGTCCACCGGGTTTTATCAA	22	20	36.4	50.0	54.8	58.2	0.0	6.3	5.360309	119663	119663	151-151	1.000000	40.5
ndhA-ndhH_p4	ndhA-ndhH	4	TGATATCTTGGACTTCTGTTGT	CGTCCACCGGGTTTTATCAA	22	20	36.4	50.0	54.8	58.2	4.0	6.3	5.360309	119663	119663	155-155	1.000000	40.5
ndhA-ndhH_p5	ndhA-ndhH	5	TCTTGGACTTCTGTTGTATCAAT	CGTCCACCGGGTTTTATCAA	23	20	34.8	50.0	55.6	58.2	0.0	6.3	5.594735	119663	119663	150-150	1.000000	40.5
rps15-ycf1_p1	rps15-ycf1	1	TCAACTGATCCGGGGTTTTC	TCCACGGTTGAAAATACGGT	20	20	50.0	45.0	57.8	57.5	0.0	0.0	0.741501	121223	121596	463-476	1.000000	77.8
rps15-ycf1_p2	rps15-ycf1	2	CAACTGATCCGGGGTTTTCT	TCCACGGTTGAAAATACGGT	20	20	50.0	45.0	57.8	57.5	0.0	0.0	0.747672	121223	121596	462-475	1.000000	77.8
rps15-ycf1_p3	rps15-ycf1	3	ACTGATCCGGGGTTTTCTTC	TCCACGGTTGAAAATACGGT	20	20	50.0	45.0	57.5	57.5	0.0	0.0	1.038974	121223	121596	460-473	1.000000	75.5
rps15-ycf1_p4	rps15-ycf1	4	TTCAACTGATCCGGGGTTTT	TCCACGGTTGAAAATACGGT	20	20	45.0	45.0	57.3	57.5	0.0	0.0	1.215443	121223	121596	464-477	1.000000	74.0
rps15-ycf1_p5	rps15-ycf1	5	TCAACTGATCCGGGGTTTTC	ATCCACGGTTGAAAATACGGT	20	21	50.0	42.9	57.8	57.9	0.0	0.0	1.256324	121223	121596	464-477	1.000000	73.7
ycf1_p1	ycf1	1	TCAATTTCACTCCTTTCTTTAAT	AGCTATTCGATCTAATAAGGG	23	21	26.1	38.1	52.3	52.0	0.0	1.8	15.699502	126230	126349	165-165	1.000000	46.8
ycf1_p2	ycf1	2	TCAATTTCACTCCTTTCTTTAATT	AGCTATTCGATCTAATAAGGG	24	21	25.0	38.1	53.1	52.0	0.0	1.8	15.856270	126230	126349	165-165	1.000000	46.8
ycf1_p3	ycf1	3	TCAATTTCACTCCTTTCTTTAAT	GTAGCTATTCGATCTAATAAGGG	23	23	26.1	39.1	52.3	53.7	0.0	0.0	15.990185	126230	126349	167-167	1.000000	46.7
ycf1_p4	ycf1	4	TCAATTTCACTCCTTTCTTTAATT	GTAGCTATTCGATCTAATAAGGG	24	23	25.0	39.1	53.1	53.7	0.0	0.0	16.146952	126230	126349	167-167	1.000000	46.7
ycf1_p5	ycf1	5	TCAATTTCACTCCTTTCTTTAAT	TAGCTATTCGATCTAATAAGGG	23	22	26.1	36.4	52.3	52.2	0.0	1.8	16.586409	126230	126349	166-166	1.000000	46.7