Hotspot

rps2

LSC · 15882-16592 · 711 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps2

Feature Metadata

label_name
rps2
feature_type
gene
region
LSC
length_bp
711
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2_p1 TGCAAGACAAGACATAGTAACCA GGCTGTGTATCAACGGTCAA 39.1 50.0 57.8 58.2 872 bp 1.0 52.7
rps2_p2 TGCAAGACAAGACATAGTAACCA CAATCCCGGTAGAAGGTTCC 39.1 55.0 57.8 57.7 855 bp 1.0 51.6
rps2_p3 TGCAAGACAAGACATAGTAACCA TTTGGGCTGTGTATCAACGG 39.1 50.0 57.8 58.5 876 bp 1.0 50.5
rps2_p4 TGCAAGACAAGACATAGTAACCA GTTTGGGCTGTGTATCAACG 39.1 50.0 57.8 57.4 877 bp 1.0 49.3
rps2_p5 TGCAAGACAAGACATAGTAACCA GGTCAATCCCGGTAGAAGGT 39.1 55.0 57.8 58.8 858 bp 1.0 47.8