Hotspot

rps16

LSC · 4621-5757 · 1137 bp

Back to Acis

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1137
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 AGAAAGGAGCAAATTGTGGGT ACCGTTGAACCAATGACTACT 42.9 42.9 58.0 57.2 1313 bp 1.0 57.6
rps16_p2 GAAAGGAGCAAATTGTGGGT ACCGTTGAACCAATGACTACT 45.0 42.9 56.5 57.2 1312 bp 1.0 53.9
rps16_p3 AGAAAGGAGCAAATTGTGGGT ACCGTTGAACCAATGACTAC 42.9 45.0 58.0 55.7 1313 bp 1.0 53.3
rps16_p4 AGAAAGGAGCAAATTGTGGGT ACCGTTGAACCAATGACTACTC 42.9 45.5 58.0 58.3 1313 bp 1.0 53.0
rps16_p5 AGAAAGGAGCAAATTGTGGGT CCGTTGAACCAATGACTACTCA 42.9 45.5 58.0 58.3 1312 bp 1.0 53.0