Hotspot

psbA

LSC · 67-1128 · 1062 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 AGGTATAGCTCCTTCAACGA GTTAGTATGCGTGCTTGGGA 45.0 50.0 55.4 58.0 1160 bp 1.0 58.8
psbA_p2 AGGTATAGCTCCTTCAACGA GTGCTTGGGAGTCCTTGAAA 45.0 50.0 55.4 58.0 1150 bp 1.0 58.8
psbA_p3 AGGTATAGCTCCTTCAACGA AGTTAGTATGCGTGCTTGGG 45.0 50.0 55.4 58.0 1161 bp 1.0 58.8
psbA_p4 AAGGTATAGCTCCTTCAACGA GTTAGTATGCGTGCTTGGGA 42.9 50.0 56.2 58.0 1161 bp 1.0 57.3
psbA_p5 AAGGTATAGCTCCTTCAACGA GTGCTTGGGAGTCCTTGAAA 42.9 50.0 56.2 58.0 1151 bp 1.0 57.3