Hotspot

atpI

LSC · 14899-15642 · 744 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
LSC
length_bp
744
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 CATGCATTGCCTCAATTGGG AGATTTCTGCCAGAGGACAA 50.0 45.0 58.1 56.4 822 bp 1.0 67.1
atpI_p2 CCATGCATTGCCTCAATTGG AGATTTCTGCCAGAGGACAA 50.0 45.0 58.1 56.4 823 bp 1.0 67.1
atpI_p3 ATGCATTGCCTCAATTGGGA AGATTTCTGCCAGAGGACAA 45.0 45.0 58.1 56.4 821 bp 1.0 66.5
atpI_p4 TGCATTGCCTCAATTGGGAT AGATTTCTGCCAGAGGACAA 45.0 45.0 58.1 56.4 820 bp 1.0 66.5
atpI_p5 TGAACCATGCATTGCCTCAA AGATTTCTGCCAGAGGACAA 45.0 45.0 58.4 56.4 827 bp 1.0 64.6