Hotspot

atpF

LSC · 12341-13755 · 1415 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1415
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 ACTAATTTCGTCAGCTCGAA GGCGAGCCAAGTGATACAAA 40.0 50.0 54.9 58.6 1582 bp 1.0 50.4
atpF_p2 ACTAATTTCGTCAGCTCGAA GGCGAGCCAAGTGATACAA 40.0 52.6 54.9 57.8 1582 bp 1.0 45.6
atpF_p3 ACTAATTTCGTCAGCTCGAA GCGAGCCAAGTGATACAAAA 40.0 45.0 54.9 56.7 1581 bp 1.0 44.6
atpF_p4 ACTAATTTCGTCAGCTCGAA AAAGGGCGAGCCAAGTGATA 40.0 50.0 54.9 59.4 1586 bp 1.0 43.8
atpF_p5 TACTAATTTCGTCAGCTCGA GGCGAGCCAAGTGATACAAA 40.0 50.0 54.0 58.6 1583 bp 1.0 43.5