Hotspot

atpA

LSC · 10744-12267 · 1524 bp

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TCGAATCAGAAATAGTTCGCA	CTTGGGGCCATGGAAGAAAT	38.1	50.0	55.5	58.1	1689 bp	1.0	50.6
atpA_p2	TCGAATCAGAAATAGTTCGCAA	CTTGGGGCCATGGAAGAAAT	36.4	50.0	56.2	58.1	1689 bp	1.0	48.6
atpA_p3	TTCGAATCAGAAATAGTTCGCA	CTTGGGGCCATGGAAGAAAT	36.4	50.0	56.2	58.1	1690 bp	1.0	48.6
atpA_p4	GGCGATACCAAGAAACTTTT	CTTGGGGCCATGGAAGAAAT	40.0	50.0	54.2	58.1	1721 bp	1.0	48.5
atpA_p5	AGTTCGCAATGATAACTCTTGT	CTTGGGGCCATGGAAGAAAT	36.4	50.0	56.1	58.1	1676 bp	1.0	47.8