Hotspot

trnS-GCU-trnG-GCC

LSC · 8205-9644 · 1440 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1440
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 ATTAGCAATCCGCCGCTTTA CGAACGAATCACACTTTTACCA 45.0 40.9 58.0 57.3 1517 bp 1.0 57.9
trnS-GCU-trnG-GCC_p2 ATTAGCAATCCGCCGCTTTA AGACCGAAAGATCCCTTAACT 45.0 42.9 58.0 56.1 1553 bp 1.0 56.3
trnS-GCU-trnG-GCC_p3 ATTAGCAATCCGCCGCTTTA TCGAACGAATCACACTTTTACC 45.0 40.9 58.0 57.0 1518 bp 1.0 55.9
trnS-GCU-trnG-GCC_p4 CGGAGAGAGAGGGATTCGAA CGAACGAATCACACTTTTACCA 55.0 40.9 58.3 57.3 1565 bp 1.0 55.7
trnS-GCU-trnG-GCC_p5 GCTTTAGTCCACTCAGCCAT CGAACGAATCACACTTTTACCA 50.0 40.9 57.6 57.3 1503 bp 1.0 55.0