Hotspot

rps16-trnQ-UUG

LSC · 5758-6866 · 1109 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1109
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCACAGCGAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1205 bp 1.0 79.1
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1182 bp 1.0 78.3
rps16-trnQ-UUG_p3 TGCTTTCTACCGCATCGTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.2 58.1 1171 bp 1.0 77.8
rps16-trnQ-UUG_p4 TTGCTTTCTACCGCATCGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.2 58.1 1172 bp 1.0 77.8
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1193 bp 1.0 77.8