Hotspot

trnK-UUU-rps16

LSC · 4021-4620 · 600 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
600
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG GGTGCTCAACCTACAGGAAC 50.0 55.0 58.6 58.2 717 bp 1.0 73.9
trnK-UUU-rps16_p2 TAGTCGCACTTAAAAGCCGA GGTGCTCAACCTACAGGAAC 45.0 55.0 57.3 58.2 718 bp 1.0 72.6
trnK-UUU-rps16_p3 TCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 45.0 55.0 57.3 58.2 715 bp 1.0 72.6
trnK-UUU-rps16_p4 CGCACTTAAAAGCCGAGTAC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.2 58.2 714 bp 1.0 72.2
trnK-UUU-rps16_p5 GTCGCACTTAAAAGCCGAGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.9 58.2 716 bp 1.0 71.6