Hotspot

rps18

LSC · 68244-68735 · 492 bp

Back to Acidosasa

Pi 0.00136

MarkerSeek score 44.90

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 492
variable_sites: 1
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AGACTACTTCTTCTGGATCCA	GGTCTGGCCCTTTCGAATAA	42.9	50.0	55.1	57.6	596-597 bp	1.0	46.0
rps18_p2	AGACTACTTCTTCTGGATCCA	AGGTCTGGCCCTTTCGAATA	42.9	50.0	55.1	58.4	597-598 bp	1.0	46.0
rps18_p3	AGACTACTTCTTCTGGATCCA	ATAGGTCTGGCCCTTTCGAA	42.9	50.0	55.1	58.4	599-600 bp	1.0	46.0
rps18_p4	AGACTACTTCTTCTGGATCCA	TAGGTCTGGCCCTTTCGAAT	42.9	50.0	55.1	58.4	598-599 bp	1.0	46.0
rps18_p5	TGGACCAAAGAAAGACTACTTCT	GGTCTGGCCCTTTCGAATAA	39.1	50.0	57.1	57.6	608-609 bp	1.0	45.8