Hotspot

matK

LSC · 1708-3243 · 1536 bp

Back to Acidosasa

Pi 0.000434

MarkerSeek score 46.50

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1536
variable_sites: 1
pi: 0.000434
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCAGAAGAACATTTCAGAATGGA	ATGCAACACCCTGTTTTGAC	34.8	45.0	55.9	57.1	1715 bp	1.0	40.0
matK_p2	TCAGAAGAACATTTCAGAATGGA	AATGCAACACCCTGTTTTGAC	34.8	42.9	55.9	57.8	1716 bp	1.0	40.0
matK_p3	TCAGAAGAACATTTCAGAATGGA	GCAACACCCTGTTTTGACCA	34.8	50.0	55.9	59.2	1713 bp	1.0	40.0
matK_p4	TCAGAAGAACATTTCAGAATGGA	TGCAACACCCTGTTTTGACC	34.8	50.0	55.9	59.2	1714 bp	1.0	40.0
matK_p5	TCAGAAGAACATTTCAGAATGGAA	ATGCAACACCCTGTTTTGAC	33.3	45.0	56.5	57.1	1715 bp	1.0	40.0